Análisis Cuantitativo de la Estructura de Fibras Colágenas en Útero Canino Expuesto a Acetato de Medroxiprogesterona Utilizando Generación Microscópica de un Segundo Harmónico

Paulo Salinas, Maria Angélica Miglino, Mariano Del Sol

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

Resumen

Collagen is the most abundant structural protein found in organs and is responsible for providing tissues with structure and function. In order to investigate in canine uteri the potential effect of medroxyprogesterone acetate (MPA) on the changes in collagen deposition were grouped as nulliparous (n=11), multiparous (n=11) and treated with MPA (n=11; nulliparous with two treatments; 5 mg/kg; i.m.). The amount of collagen was studied in the fold and basal regions of the endometrium and myometrium using second harmonic generation with a two-photon spectral confocal microscope, quantified using ImageJ software with a color segmentation plugin, was expressed as fraction area (%) and analyzed by ANOVA (p<0,05). No differences were observed between groups in the fold (p=0,3995) or base (p=0,7392) of the endometrium and myometrium (p=0,1781). In conclusion, our data demonstrate that two doses of MPA (5 mg/kg; i.m.) do not affect the total collagen deposition in canine uteri undergoing contraceptive treatment.

Título traducido de la contribuciónQuantitative analysis of collagen fibrillar structure in canine uterus exposed to acetate of medroxyprogesterone using second harmonic generation microscopy
Idioma originalEspañol
Páginas (desde-hasta)157-161
Número de páginas5
PublicaciónInternational Journal of Morphology
Volumen35
N.º1
DOI
EstadoPublicada - 2017
Publicado de forma externa

Palabras clave

  • Canine
  • Collagen
  • Medroxyprogesterone acetate
  • Second harmonic generation

Huella

Profundice en los temas de investigación de 'Análisis Cuantitativo de la Estructura de Fibras Colágenas en Útero Canino Expuesto a Acetato de Medroxiprogesterona Utilizando Generación Microscópica de un Segundo Harmónico'. En conjunto forman una huella única.

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