An immunological method for quantifying antibacterial activity in Salmo salar (Linnaeus, 1758) skin mucus

Edgar Narvaez; Jorge Berendsen; Fanny Guzmán; José A A. Gallardo; Luis Mercado

doi:10.1016/j.fsi.2009.10.004

An immunological method for quantifying antibacterial activity in Salmo salar (Linnaeus, 1758) skin mucus

Edgar Narvaez, Jorge Berendsen, Fanny Guzmán, José A A. Gallardo, Luis Mercado

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25 Citas (Scopus)

Resumen

Antimicrobial peptides (AMPs) are a pivotal component of innate immunity in lower vertebrates. The aim of this study was to develop an immunological method for quantifying AMPs in Salmo salar skin mucus. A known antimicrobial peptide derived from histone H1 previously purified and described from S. salar skin mucus (SAMP H1) was chemically synthesized and used to obtain antibodies for the quantification of the molecule via ELISA. Using skin mucus samples, a correlation of bacterial growth inhibition versus SAMP H1 concentration (ELISA) was established. The results provide the first evidence for quantifying the presence of active AMPs in the skin mucus of S. salar through the use of an immunological method.

Idioma original	Inglés
Páginas (desde-hasta)	235-239
Número de páginas	5
Publicación	Fish and Shellfish Immunology
Volumen	28
N.º	1
DOI	https://doi.org/10.1016/j.fsi.2009.10.004
Estado	Publicada - ene. 2010

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title = "An immunological method for quantifying antibacterial activity in Salmo salar (Linnaeus, 1758) skin mucus",

abstract = "Antimicrobial peptides (AMPs) are a pivotal component of innate immunity in lower vertebrates. The aim of this study was to develop an immunological method for quantifying AMPs in Salmo salar skin mucus. A known antimicrobial peptide derived from histone H1 previously purified and described from S. salar skin mucus (SAMP H1) was chemically synthesized and used to obtain antibodies for the quantification of the molecule via ELISA. Using skin mucus samples, a correlation of bacterial growth inhibition versus SAMP H1 concentration (ELISA) was established. The results provide the first evidence for quantifying the presence of active AMPs in the skin mucus of S. salar through the use of an immunological method.",

keywords = "Antibacterial quantification, Fish antibacterial peptide, SAMP H1, Salmo salar",

author = "Edgar Narvaez and Jorge Berendsen and Fanny Guzm{\'a}n and Gallardo, {Jos{\'e} A A.} and Luis Mercado",

year = "2010",

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doi = "10.1016/j.fsi.2009.10.004",

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T1 - An immunological method for quantifying antibacterial activity in Salmo salar (Linnaeus, 1758) skin mucus

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AU - Gallardo, José A A.

AU - Mercado, Luis

PY - 2010/1

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N2 - Antimicrobial peptides (AMPs) are a pivotal component of innate immunity in lower vertebrates. The aim of this study was to develop an immunological method for quantifying AMPs in Salmo salar skin mucus. A known antimicrobial peptide derived from histone H1 previously purified and described from S. salar skin mucus (SAMP H1) was chemically synthesized and used to obtain antibodies for the quantification of the molecule via ELISA. Using skin mucus samples, a correlation of bacterial growth inhibition versus SAMP H1 concentration (ELISA) was established. The results provide the first evidence for quantifying the presence of active AMPs in the skin mucus of S. salar through the use of an immunological method.

AB - Antimicrobial peptides (AMPs) are a pivotal component of innate immunity in lower vertebrates. The aim of this study was to develop an immunological method for quantifying AMPs in Salmo salar skin mucus. A known antimicrobial peptide derived from histone H1 previously purified and described from S. salar skin mucus (SAMP H1) was chemically synthesized and used to obtain antibodies for the quantification of the molecule via ELISA. Using skin mucus samples, a correlation of bacterial growth inhibition versus SAMP H1 concentration (ELISA) was established. The results provide the first evidence for quantifying the presence of active AMPs in the skin mucus of S. salar through the use of an immunological method.

KW - Antibacterial quantification

KW - Fish antibacterial peptide

KW - SAMP H1

KW - Salmo salar

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An immunological method for quantifying antibacterial activity in Salmo salar (Linnaeus, 1758) skin mucus

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