TY - JOUR
T1 - Antimicrobial and degradable triazolinedione (TAD) crosslinked polypeptide hydrogels
AU - KIMMINS, SCOTT DAVID
AU - Hanay, Saltuk B.
AU - Murphy, Robert
AU - O’Dwyer, Joanne
AU - Ramalho, Jessica
AU - Ryan, Emily J.
AU - Kearney, Cathal J.
AU - O'Brien, Fergal J.
AU - Cryan, Sally Ann
AU - Fitzgerald-Hughes, Deirdre
AU - Heise, Andreas
N1 - Funding Information:
This project has received funding from the Translational Research in Nanomedical Devices (TREND) project, Science Foundation Ireland Investigators Program (Grant code 13/IA/ 1840(T)) and ANID-Fondecyt project no. 11190462.
Publisher Copyright:
© The Royal Society of Chemistry 2021.
PY - 2021/7/21
Y1 - 2021/7/21
N2 - Hydrogels are perfectly suited to support cell and tissue growth in advanced tissue engineering applications as well as classical wound treatment scenarios. Ideal hydrogel materials for these applications should be easy to produce, biocompatible, resorbable and antimicrobial. Here we report the fabrication of degradable covalent antimicrobial lysine and tryptophan containing copolypeptide hydrogels, whereby the hydrogel properties can be independently modulated by the copolypeptide monomer ratio and chiral composition. Well-defined statistical copolypeptides comprising different overall molecular weights as well as ratios ofl- andd-lysine and tryptophan at ratios of 35 : 15, 70 : 30 and 80 : 20 were obtained byN-carboxyanhydride (NCA) polymerisation and subsequently crosslinked by the selective reaction of bifunctional triazolinedione (TAD) with tryptophan. Real-time rheology was used to monitor the crosslinking reaction recording the fastest increase and overall modulus for copolypeptides with the higher tryptophan ratio. Water uptake of cylindrical hydrogel samples was dependent on crosslinking ratio but found independent of chiral composition, while enzymatic degradation proceeded significantly faster for samples containing morel-amino acids. Antimicrobial activity on a range of hydrogels containing different polypeptide chain lengths, lysine/tryptophan composition andl/denantiomers was tested against reference laboratory strains of Gram-negativeEscherichia coli(E. coli; ATCC25922) and Gram-positive,Staphylococcus aureus(S. aureus; ATCC25923). log reductions of 2.8-3.4 were recorded for the most potent hydrogels.In vitroleachable cytotoxicity tests confirmed non-cytotoxicity as per ISO guidelines.
AB - Hydrogels are perfectly suited to support cell and tissue growth in advanced tissue engineering applications as well as classical wound treatment scenarios. Ideal hydrogel materials for these applications should be easy to produce, biocompatible, resorbable and antimicrobial. Here we report the fabrication of degradable covalent antimicrobial lysine and tryptophan containing copolypeptide hydrogels, whereby the hydrogel properties can be independently modulated by the copolypeptide monomer ratio and chiral composition. Well-defined statistical copolypeptides comprising different overall molecular weights as well as ratios ofl- andd-lysine and tryptophan at ratios of 35 : 15, 70 : 30 and 80 : 20 were obtained byN-carboxyanhydride (NCA) polymerisation and subsequently crosslinked by the selective reaction of bifunctional triazolinedione (TAD) with tryptophan. Real-time rheology was used to monitor the crosslinking reaction recording the fastest increase and overall modulus for copolypeptides with the higher tryptophan ratio. Water uptake of cylindrical hydrogel samples was dependent on crosslinking ratio but found independent of chiral composition, while enzymatic degradation proceeded significantly faster for samples containing morel-amino acids. Antimicrobial activity on a range of hydrogels containing different polypeptide chain lengths, lysine/tryptophan composition andl/denantiomers was tested against reference laboratory strains of Gram-negativeEscherichia coli(E. coli; ATCC25922) and Gram-positive,Staphylococcus aureus(S. aureus; ATCC25923). log reductions of 2.8-3.4 were recorded for the most potent hydrogels.In vitroleachable cytotoxicity tests confirmed non-cytotoxicity as per ISO guidelines.
UR - http://www.scopus.com/inward/record.url?scp=85110436159&partnerID=8YFLogxK
U2 - 10.1039/d1tb00776a
DO - 10.1039/d1tb00776a
M3 - Article
AN - SCOPUS:85110436159
SN - 2050-750X
VL - 9
SP - 5456
EP - 5464
JO - Journal of Materials Chemistry B
JF - Journal of Materials Chemistry B
IS - 27
ER -