Co-feeding strategy alleviates hypoxic stress in large-scale bioreactor for optimal production of secretory recombinant proteins in Pichia pastoris

Edgar Velastegui, Johan Quezada, Claudia Altamirano, Julio Berrios, Patrick Fickers

Producción científica: Contribución a una revistaArtículorevisión exhaustiva

Resumen

The loss of mixing efficiency inherent to bioreactor process operated at large-scale yields to the formation of concentration gradient and thus to heterogeneous culture conditions. For processes operated with methanol feeding, P. pastoris faces oscillatory culture conditions that significantly affect the cell ability to produce secretory recombinant proteins at high yield. Extended cell residence time in microenvironments of high methanol concentration and low oxygen availability that are typically found in the upper part of the bioreactor near the feeding point, triggers the unfolded protein response (UPR) and thus impairs proper protein secretion. Methanol co-feeding with sorbitol was shown herein to reduce the UPR response and to restore productivity of secreted protein.

Idioma originalInglés
Páginas (desde-hasta)20-23
Número de páginas4
PublicaciónJournal of Biotechnology
Volumen373
DOI
EstadoPublicada - 20 ago. 2023

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Profundice en los temas de investigación de 'Co-feeding strategy alleviates hypoxic stress in large-scale bioreactor for optimal production of secretory recombinant proteins in Pichia pastoris'. En conjunto forman una huella única.

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