TY - JOUR
T1 - Co-immobilized carrier-free enzymes for lactose upgrading
AU - Wilson, Lorena
AU - Illanes, Andrés
AU - Ottone, Carminna
AU - Romero, Oscar
N1 - Funding Information:
This work was supported by FONDECYT-Chile (Grant number 1190709 ).
Publisher Copyright:
© 2021 Elsevier B.V.
PY - 2022/2
Y1 - 2022/2
N2 - The enzymatic production of lactofructose syrup from lactose coming from surplus whey represents a sound example of the circular economy. The production process requires the sequential action of two enzymes, namely β-galactosidase and glucose (xylose) isomerase, which can act independently in different reactors or a one-pot system. The latter is an interesting option, even more, if considering the co-immobilization of the enzymes involved in the biotransformation. This review analyzes the different options to carry out this process, with a focus on the key aspects of the co-immobilization of the enzymes in a carrier-free system and the operating conditions for such catalysts, which are key issues for developing a successful technology. The production of lactofructose syrup from lactose is an illustrative case study, but the analysis performed both on the preparation and use of the biocatalyst can be extrapolated to other co-immobilized multienzyme systems.
AB - The enzymatic production of lactofructose syrup from lactose coming from surplus whey represents a sound example of the circular economy. The production process requires the sequential action of two enzymes, namely β-galactosidase and glucose (xylose) isomerase, which can act independently in different reactors or a one-pot system. The latter is an interesting option, even more, if considering the co-immobilization of the enzymes involved in the biotransformation. This review analyzes the different options to carry out this process, with a focus on the key aspects of the co-immobilization of the enzymes in a carrier-free system and the operating conditions for such catalysts, which are key issues for developing a successful technology. The production of lactofructose syrup from lactose is an illustrative case study, but the analysis performed both on the preparation and use of the biocatalyst can be extrapolated to other co-immobilized multienzyme systems.
KW - Biocatalysis
KW - Combi-CLEAs
KW - Enzyme co-immobilization
KW - Enzyme reactor
KW - Lactofructose syrup
KW - Reactor operation conditions
UR - http://www.scopus.com/inward/record.url?scp=85115763926&partnerID=8YFLogxK
U2 - 10.1016/j.cogsc.2021.100553
DO - 10.1016/j.cogsc.2021.100553
M3 - Review article
AN - SCOPUS:85115763926
VL - 33
JO - Current Opinion in Green and Sustainable Chemistry
JF - Current Opinion in Green and Sustainable Chemistry
SN - 2452-2236
M1 - 100553
ER -