TY - JOUR
T1 - Composition control of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) copolymerization by oxygen transfer rate (OTR) in Azotobacter vinelandii OPNA
AU - Torres-Pedraza, Angie Johanna
AU - Salgado-Lugo, Holjes
AU - Segura, Daniel
AU - DIAZ BARRERA, ALVARO ENRIQUE
AU - Peña, Carlos
N1 - Funding Information:
The authors thank Dr Celia Flores for contributing their knowledge and advice towards the development of this study. This work was financed by PAPIIT (IG200219) and CONACYT‐DFG 277600.
Publisher Copyright:
© 2021 Society of Chemical Industry (SCI).
PY - 2021
Y1 - 2021
N2 - BACKGROUND: The aim of this study was to evaluate changes in the composition of poly-3-hydoxybutyrate-co-3-hydroxyvalerate (PHBV) by the polyhydroxybutyrate (PHB) overproducing mutant OPNA of Azotobacter vinelandii cultured under different conditions. RESULTS: The oxygen transfer rate (OTR) significantly affected PHBV composition in cultures in shaken flasks; the highest content of 3-hydroxyvalerate (3HV; 28%) was achieved from cultures developed at an OTRmax of 5.87 mmol L−1 h−1. In the bioreactor, cultures were grown under oxygen-limited conditions and different OTRs, varying the agitation rate. The highest PHBV accumulation (85%) was achieved at a low OTRmax (4.96 mmol L−1 h −1), whereas the maximum content of 3HV (8.5 mol%) was obtained at a higher OTRmax (20.3 mmol L−1 h−1). The OPNA strain produced a copolymer of ultra-high molecular weight. CONCLUSIONS: Our results reveal that increasing the OTRmax raises the molar fraction of 3HV in the PHBV copolymer. Therefore, the manipulation of OTR could be a feasible strategy to produce PHBV copolymers with different monomeric compositions at industrial level.
AB - BACKGROUND: The aim of this study was to evaluate changes in the composition of poly-3-hydoxybutyrate-co-3-hydroxyvalerate (PHBV) by the polyhydroxybutyrate (PHB) overproducing mutant OPNA of Azotobacter vinelandii cultured under different conditions. RESULTS: The oxygen transfer rate (OTR) significantly affected PHBV composition in cultures in shaken flasks; the highest content of 3-hydroxyvalerate (3HV; 28%) was achieved from cultures developed at an OTRmax of 5.87 mmol L−1 h−1. In the bioreactor, cultures were grown under oxygen-limited conditions and different OTRs, varying the agitation rate. The highest PHBV accumulation (85%) was achieved at a low OTRmax (4.96 mmol L−1 h −1), whereas the maximum content of 3HV (8.5 mol%) was obtained at a higher OTRmax (20.3 mmol L−1 h−1). The OPNA strain produced a copolymer of ultra-high molecular weight. CONCLUSIONS: Our results reveal that increasing the OTRmax raises the molar fraction of 3HV in the PHBV copolymer. Therefore, the manipulation of OTR could be a feasible strategy to produce PHBV copolymers with different monomeric compositions at industrial level.
KW - Azotobacter vinelandii
KW - monomeric composition
KW - oxygen transfer rate
KW - poly(3-hydroxybutyrate-co-3-hydroxyvalerate)
KW - valeric acid
UR - http://www.scopus.com/inward/record.url?scp=85108842197&partnerID=8YFLogxK
U2 - 10.1002/jctb.6825
DO - 10.1002/jctb.6825
M3 - Article
AN - SCOPUS:85108842197
JO - Journal of Chemical Technology and Biotechnology
JF - Journal of Chemical Technology and Biotechnology
SN - 0268-2575
ER -