Digital holography microscopy in 3D biologic samples analysis

J. O. Ricardo, M. Muramatsu, F. Palacios, M. Gesualdi, O. Font, J. L. Valin, M. Escobedo, S. Herold, D. F. Palacios, G. F. Palacios, A. Sánchez

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Resumen

In this work it is used a setup for Digital Holography Microscopy (MHD) for 3D biologic samples reconstruction. The phase contrast image reconstruction is done by using the Double propagation Method. The system was calibrated and tested by using a micrometric scale and pure phase object respectively. It was simulated the human red blood cell (erythrocyte) and beginning from the simulated hologram the digital 3D phase image for erythrocytes it was calculated. Also there was obtained experimental holograms of human erythrocytes and its corresponding 3D phase images, being evident the correspondence qualitative and quantitative between these characteristics in the simulated erythrocyte and in the experimentally calculated by DHM in both cases.

Idioma originalInglés
Número de artículo012066
PublicaciónJournal of Physics: Conference Series
Volumen274
N.º1
DOI
EstadoPublicada - 2011
Publicado de forma externa

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