TY - JOUR
T1 - Estimating the product inhibition constant from enzyme kinetic equations using the direct linear plot method in one-stage treatment
AU - Valencia, Pedro L.
AU - Sepúlveda, Bastián
AU - Gajardo, Diego
AU - Astudillo-Castro, Carolina
N1 - Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2020/8
Y1 - 2020/8
N2 - A direct linear plot was applied to estimate kinetic constants using the product’s competitive inhibition equation. The challenge consisted of estimating three kinetic constants, Vmax, Km, and Kp, using two independent variables, substrates, and product concentrations, in just one stage of mathematical treatment. The method consisted of combining three initial reaction rate data and avoiding the use of the same three product concentrations (otherwise, this would result in a mathematical indetermination). The direct linear plot method was highly superior to the least-squares method in terms of accuracy and robustness, even under the addition of error. The direct linear plot method is a reliable and robust method that can be applied to estimate Kp in inhibition studies in pharmaceutical and biotechnological areas.
AB - A direct linear plot was applied to estimate kinetic constants using the product’s competitive inhibition equation. The challenge consisted of estimating three kinetic constants, Vmax, Km, and Kp, using two independent variables, substrates, and product concentrations, in just one stage of mathematical treatment. The method consisted of combining three initial reaction rate data and avoiding the use of the same three product concentrations (otherwise, this would result in a mathematical indetermination). The direct linear plot method was highly superior to the least-squares method in terms of accuracy and robustness, even under the addition of error. The direct linear plot method is a reliable and robust method that can be applied to estimate Kp in inhibition studies in pharmaceutical and biotechnological areas.
KW - Direct linear plot
KW - Distribution-free method
KW - Kinetic constants
KW - Median method
KW - Non-parametric
KW - Product inhibition
UR - http://www.scopus.com/inward/record.url?scp=85088976611&partnerID=8YFLogxK
U2 - 10.3390/catal10080853
DO - 10.3390/catal10080853
M3 - Article
AN - SCOPUS:85088976611
VL - 10
JO - Catalysts
JF - Catalysts
SN - 2073-4344
IS - 8
M1 - 853
ER -