Immobilization of lactase for the continuous hydrolysis of whey permaete

A. Illanes, A. Ruiz, M. E. Zúñiga, C. Aguirre, S. O'Reilly, E. Curotto

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

40 Citas (Scopus)

Resumen

The production of lactose-based sweeteners is considered very promising. Fungal lactase has been immobilized on crosslinked chitin to develop a process for the continuous hydrolysis of demineralized whey permaete. The optimization of lactase immobilization on chitin and chitosan was performed, activities of 4 · 105 and 2.2 · 105 u/kg at yields of 33 and 23% were obtained for both supports, respectively. The chitin based catalyst was selected for further studies and a procedure was developed for in-situ enzyme immobilization. The kinetic behaviour of the catalyst was determined to propose a kinetic model for the initial rate of lactose hydrolysis. Pseudo steady-state and long term operation of packed bed reactors with chitin-immobilized lactase ranging from small laboratory to pre-pilot unit was carried out. The results are discussed and compared with commercial immobilized lactases. Preliminary economic evaluation for the production of ultrafiltered whey protein and hydrolyzed lactose syrup, within a dairy industry in Chile, was satisfactory in terms of profitability, both for the chitin immobilized lactase developed and for a commercial immobilized lactase.

Idioma originalInglés
Páginas (desde-hasta)257-262
Número de páginas6
PublicaciónBioprocess Engineering
Volumen5
N.º6
DOI
EstadoPublicada - nov. 1990
Publicado de forma externa

Huella

Profundice en los temas de investigación de 'Immobilization of lactase for the continuous hydrolysis of whey permaete'. En conjunto forman una huella única.

Citar esto