TY - JOUR
T1 - In vitro growth of ectomycorrhizal fungi associated with Pinus radiata plantations in Chile
AU - García-Rodríguez, José Leonardo
AU - Pérez-Moreno, Jesús
AU - Ríos-Leal, Darcy
AU - Saez-Delgado, Patricia
AU - Atala-Bianchi, Cristian
AU - Sánchez-Olate, Manuel
AU - Pereira-Cancino, Guillermo
N1 - Funding Information:
The support of the National Institute of Forestry, Agriculture and Livestock Research of México (INIFAP, CORECADE NC13/83), of The Council National of Science and Technology of México (CONACYT 172007), and of The Chilean National Council for Science and Technology (CONICYT 2115253) through a doctoral scholarship granted to J. L. García-Rodríguez is acknowledged. The authors also thank two anonymous referees and the Editor whose valuable comments helped to substantially improve the original manuscript.
Funding Information:
The support of the National Institute of Forestry, Agriculture and Livestock Research of M?xico (INIFAP, CORECADE NC13/83), of The Council National of Science and Technology of M?xico (CONACYT 172007), and of The Chilean National Council for Science and Technology (CONICYT 2115253) through a doctoral scholarship granted to J.L. Garc?a-Rodr?guez is acknowledged. The authors also thank two anonymous referees and the Editor whose valuable comments helped to substantially improve the original manuscript.
PY - 2017
Y1 - 2017
N2 - A comparative study of in vitro growth of three species of ectomycorrhizal fungi (ECMF) (Rhizopogon luteolus, Suillus granulatus and Suillus luteus) was performed. Fungal material was collected in adult Pinus radiata plantations. Isolation and purification of the strains were performed on potato-dextrose-agar medium and the evaluation of the radial growth rate and the increase in mycelial biomass, under different culture conditions, was performed on the Modified Melin Norkrans growth medium. The effects of temperature (24, 28 and 32 °C) and pH (4.8, 5.3, 5.8, 6.3 and 6.8) of the growth medium were tested for the three fungal species in two independent assays. The results indicate that the temperature had a significant effect on the radial growth rate (RG) and mycelial biomass increase (MB) in all of the evaluated fungal species. The highest RGR and MBI were recorded in R. luteolus, and the lowest values for these variables were registered in S. luteus. Rhizopogon luteolus had the highest sensitivity to pH changes. Meanwhile, there was no pattern in S. granulatus and S. luteus growth response under different pH conditions. When cultivated in vitro, the three studied species of ECMF presented adaptation, exponential, declining and stationary growth phases. The in vitro growth responses recorded in the present study showed the great potential of R. luteolus to be used in future programs using mycorrhizal inoculation in the production of Pinus radiata trees in nurseries in Chile.
AB - A comparative study of in vitro growth of three species of ectomycorrhizal fungi (ECMF) (Rhizopogon luteolus, Suillus granulatus and Suillus luteus) was performed. Fungal material was collected in adult Pinus radiata plantations. Isolation and purification of the strains were performed on potato-dextrose-agar medium and the evaluation of the radial growth rate and the increase in mycelial biomass, under different culture conditions, was performed on the Modified Melin Norkrans growth medium. The effects of temperature (24, 28 and 32 °C) and pH (4.8, 5.3, 5.8, 6.3 and 6.8) of the growth medium were tested for the three fungal species in two independent assays. The results indicate that the temperature had a significant effect on the radial growth rate (RG) and mycelial biomass increase (MB) in all of the evaluated fungal species. The highest RGR and MBI were recorded in R. luteolus, and the lowest values for these variables were registered in S. luteus. Rhizopogon luteolus had the highest sensitivity to pH changes. Meanwhile, there was no pattern in S. granulatus and S. luteus growth response under different pH conditions. When cultivated in vitro, the three studied species of ECMF presented adaptation, exponential, declining and stationary growth phases. The in vitro growth responses recorded in the present study showed the great potential of R. luteolus to be used in future programs using mycorrhizal inoculation in the production of Pinus radiata trees in nurseries in Chile.
KW - Biomass
KW - Pure culture
KW - Radial growth rate
KW - Rhizopogon
KW - Suillus
UR - http://www.scopus.com/inward/record.url?scp=85038030060&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:85038030060
SN - 0187-7380
VL - 40
SP - 415
EP - 423
JO - Revista Fitotecnia Mexicana
JF - Revista Fitotecnia Mexicana
IS - 4
ER -