Mathematical determination of kinetic parameters for assessing the effect of the organic solvent on the selectivity of peptide synthesis with immobilized α-chymotrypsin

Carola Bahamondes, Lorena Wilson, Fanny Guzmán, Andrés Illanes

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

2 Citas (Scopus)

Resumen

The synthesis of the dipeptide N-benzoyl-L-tyrosine-L-argininamide (BTAA) was conducted under kinetic control with N-benzoyl-L-tyrosine ethyl ester as acyl donor and argininamide as nucleophile using immobilized α-chymotrypsin as catalyst. Using a mathematical procedure, the kinetic constants corresponding to the proposed mechanism of peptide synthesis were determined in three different cosolvent media, namely, ethanol, diglyme and acetonitrile. These constants were used for evaluating the selectivity of glyoxyl-agarose immobilized α-chymotrypsin in the synthesis of BTAA by determining the ratios of synthesis to hydrolysis rates.

Idioma originalInglés
Páginas (desde-hasta)618-622
Número de páginas5
PublicaciónJournal of Bioscience and Bioengineering
Volumen124
N.º6
DOI
EstadoPublicada - dic. 2017
Publicado de forma externa

Huella

Profundice en los temas de investigación de 'Mathematical determination of kinetic parameters for assessing the effect of the organic solvent on the selectivity of peptide synthesis with immobilized α-chymotrypsin'. En conjunto forman una huella única.

Citar esto