Measuring β-Galactosidase activity at pH 6 with a differential pH sensor

Cristian Acevedo, Matthias H. Stach, Anette Amtmann, Manuel E. Young, Juan G. Reyes, Holger Huebner, Rainer Buchholz

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

2 Citas (Scopus)

Resumen

The β-Galactosidase activity at pH 6 is used as a cellular marker to identify senescent cell cultures. The classic method to identify this enzymatic activity is using cytochemical staining with X-Gal after 16 hrs. In this work, a differential pH sensor was used to measure β-Galactosidase activity at pH 6. The measurement is easy and only takes 3 min.

Idioma originalInglés
PublicaciónElectronic Journal of Biotechnology
Volumen12
N.º2
DOI
EstadoPublicada - 15 abr 2009
Publicado de forma externa

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