Measuring β-Galactosidase activity at pH 6 with a differential pH sensor

Cristian Acevedo; Matthias H. Stach; Anette Amtmann; Manuel E. Young; Juan G. Reyes; Holger Huebner; Rainer Buchholz

doi:10.2225/vol12-issue2-fulltext-10

Measuring β-Galactosidase activity at pH 6 with a differential pH sensor

Cristian Acevedo, Matthias H. Stach, Anette Amtmann, Manuel E. Young, Juan G. Reyes, Holger Huebner, Rainer Buchholz

Resultado de la investigación: Contribución a una revista › Artículo › revisión exhaustiva

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Resumen

The β-Galactosidase activity at pH 6 is used as a cellular marker to identify senescent cell cultures. The classic method to identify this enzymatic activity is using cytochemical staining with X-Gal after 16 hrs. In this work, a differential pH sensor was used to measure β-Galactosidase activity at pH 6. The measurement is easy and only takes 3 min.

Idioma original	Inglés
Publicación	Electronic Journal of Biotechnology
Volumen	12
N.º	2
DOI	https://doi.org/10.2225/vol12-issue2-fulltext-10
Estado	Publicada - 15 abr. 2009
Publicado de forma externa	Sí

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abstract = "The β-Galactosidase activity at pH 6 is used as a cellular marker to identify senescent cell cultures. The classic method to identify this enzymatic activity is using cytochemical staining with X-Gal after 16 hrs. In this work, a differential pH sensor was used to measure β-Galactosidase activity at pH 6. The measurement is easy and only takes 3 min.",

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AU - Amtmann, Anette

AU - Young, Manuel E.

AU - Reyes, Juan G.

AU - Huebner, Holger

AU - Buchholz, Rainer

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AB - The β-Galactosidase activity at pH 6 is used as a cellular marker to identify senescent cell cultures. The classic method to identify this enzymatic activity is using cytochemical staining with X-Gal after 16 hrs. In this work, a differential pH sensor was used to measure β-Galactosidase activity at pH 6. The measurement is easy and only takes 3 min.

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KW - Differential pH

KW - β-Galactosidase

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Measuring β-Galactosidase activity at pH 6 with a differential pH sensor

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