New strategy for the design, production and pre-purification of chimeric peptide with immunomodulatory activity in Salmo salar

Carolina Muñoz-Flores, Iván González-Chavarría, Felipe Sandoval, Francisco J. Roa, Paulo Palacios, Allisson Astuya, Khaterina Fernández, Claudia Altamirano, Alex Romero, Jannel Acosta, Jorge R. Toledo

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

Resumen

The intensive salmon farming is associated with massive outbreaks of infections. The use of antibiotics for their prevention and control is related to damage to the environment and human health. Antimicrobial peptides (AMPs) have been proposed as an alternative to the use of antibiotics for their antimicrobial and immunomodulatory activities. However, one of the main challenges for its massive clinical application is the high production cost and the complexity of chemical synthesis. Thus, recombinant DNA technology offers a more sustainable, scalable, and profitable option. In the present study, using an AMPs function prediction methodology, we designed a chimeric peptide consisting of sequences derived from cathelicidin fused with the immunomodulatory peptide derived from flagellin. The designed peptide, CATH-FLA was produced by recombinant expression using an easy pre-purification system. The chimeric peptide was able to induce IL-1β and IL-8 expression in Salmo salar head kidney leukocytes, and prevented Piscirickettsia salmonis-induced cytotoxicity in SHK-1 cells. These results suggest that pre-purification of a recombinant AMP-based chimeric peptide designed in silico allow obtaining a peptide with immunomodulatory activity in vitro. This could solve the main obstacle of AMPs for massive clinical applications.

Idioma originalInglés
Páginas (desde-hasta)120-127
Número de páginas8
PublicaciónFish and Shellfish Immunology
Volumen125
DOI
EstadoPublicada - jun. 2022
Publicado de forma externa

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