Nonchromatographic analysis of polyphenols in red wine samples: Evaluation of front-face fluorescence spectroscopy and third-order multivariate calibration to quantify catechin and epicatechin

María Elena Tarnok, Luis F. Aguilar, Manuel A. Bravo

Producción científica: Contribución a una revistaArtículorevisión exhaustiva

Resumen

In this work, an analytical approach based on third-order multivariate calibration and front-face fluorescence spectroscopy to determine the levels of (+)-catechin and (-)-epicatechin in wine samples was evaluated. An extra reaction mode (pH gradient) was incorporated into excitation-emission fluorescence second-order data, leading to higher predictivity and selectivity for catechin detection. Application of a Multivariate curve resolution-alternating least squares (MCR-ALS) algorithm resolved the systems, even in the presence of uncalibrated interferences, and yielded suitable figures of merit. Through the method, the level of (+)-catechin was directly determined, and the concentration of (-)-epicatechin was determined by the difference in total catechins (Epi + Cat) between synthetic samples. Finally, the levels of catechin were satisfactorily determined in real wine samples at mg L-1 levels with minimal sample treatment and in accordance with a reference method based on chromatographic separation.

Idioma originalInglés
Número de artículo109506
PublicaciónMicrochemical Journal
Volumen195
DOI
EstadoPublicada - dic. 2023

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