Primary cultures of hemocytes from Mytilus galloprovincialis Lmk. Expression of IL-2Rα subunit

Asunción Cao, Luis Mercado, Juan Ignacio Ramos-Martinez, Ramiro Barcia

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

70 Citas (Scopus)

Resumen

Hemocytes from the mollusk Mytilus galloprovincialis Lmk. were maintained for more than 20 days in Leibovitz L-15 medium supplemented with salts and antibiotics. Survival of cells cultured in foetal calf serum (FCS)-supplemented L-15 medium was similar to that of those cultured in inactivated hemolymph-supplemented L-15 medium. Cell activation by lipopolysaccharide (LPS) induces an increase of the number of α-subunits of the interleukin-2 (IL-2) receptor (IL-2Rα) in the cell membrane. The best response in the expression of IL-2Rα subunit was obtained after 3 days of culture. In cultured cells, as in fresh hemocytes, LPS induced a higher expression of IL-2Rα subunit than that induced by IL-2.

Idioma originalInglés
Páginas (desde-hasta)1-8
Número de páginas8
PublicaciónAquaculture
Volumen216
N.º1-4
DOI
EstadoPublicada - 10 feb 2003
Publicado de forma externa

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