Rapid Enzyme-linked immunosorbent assay for the detection of hantavirus-specific antibodies in divergent small mammals

Karla Cautivo, Tony Schountz, Mariana Acuña-Retamar, Marcela Ferrés, FERNANDO LEON TORRES PEREZ

Resultado de la investigación: Contribución a una revistaArtículorevisión exhaustiva

5 Citas (Scopus)

Resumen

We assessed the utility of an enzyme-linked immunosorbent assay (ELISA) for the detection of hantavirus-specific antibodies from sera of Oligoryzomys longicaudatus, the principal reservoir of Andes virus (ANDV), using an antigen previously developed for detection of antibodies to Sin Nombre virus (SNV) in sera from Peromyscus maniculatus. The assay uses a protein A/G horseradish peroxidase conjugate and can be performed in as little as 1.5 hours. Serum samples from Oligoryzomys longicaudatus collected in central-south Chile were used and the assay identified several that were antibody positive. This assay can be used for the rapid detection of antibodies to divergent hantaviruses from geographically and phylogenetically distant rodent species.

Idioma originalInglés
Páginas (desde-hasta)2028-2037
Número de páginas10
PublicaciónViruses
Volumen6
N.º5
DOI
EstadoPublicada - 6 may 2014

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