TY - JOUR
T1 - Relationship between tissue plasminogen activator production and specific growth rate in Chinese Hamster Ovary cells cultured in mannose at low temperature
AU - Berrios, Julio
AU - Díaz-Barrera, Alvaro
AU - Bazán, Consuelo
AU - Altamirano, Claudia
N1 - Funding Information:
Acknowledgment Authors acknowledge Projects FOND-ECYT 1070337 and DI-PUCV 203.758 for financial support, and Pharmacia (Uppsala, Sweden) for providing the cell line. Technical assistance of Verónica Figueroa and Catalina Morales is also acknowledged.
PY - 2009/8
Y1 - 2009/8
N2 - Chinese hamster ovary (CHO) cells, producing human recombinant tissue plasminogen activator (tPA), were grown with mannose (5, 20 and 40 mM) instead of glucose at 31, 33 and 37°C. The highest tPA concentration (1.5 mg l-1 at 144 h of cultivation) and tPA specific production rate (47 ng 10-6 cell h-1) were obtained at 31°C and 40 mM mannose. Regardless of the temperature or mannose concentration used, an inverse relationship between the specific growth rate and tPA specific production rate was observed, suggesting that tPA production rate would be directly controlled by the growth rate.
AB - Chinese hamster ovary (CHO) cells, producing human recombinant tissue plasminogen activator (tPA), were grown with mannose (5, 20 and 40 mM) instead of glucose at 31, 33 and 37°C. The highest tPA concentration (1.5 mg l-1 at 144 h of cultivation) and tPA specific production rate (47 ng 10-6 cell h-1) were obtained at 31°C and 40 mM mannose. Regardless of the temperature or mannose concentration used, an inverse relationship between the specific growth rate and tPA specific production rate was observed, suggesting that tPA production rate would be directly controlled by the growth rate.
KW - CHO cells
KW - Glutamate
KW - Low temperature
KW - Mannose
KW - Specific growth rate
KW - tPA
UR - http://www.scopus.com/inward/record.url?scp=70350103519&partnerID=8YFLogxK
U2 - 10.1007/s10529-009-0050-1
DO - 10.1007/s10529-009-0050-1
M3 - Article
C2 - 19547928
AN - SCOPUS:70350103519
SN - 0141-5492
VL - 31
SP - 1493
EP - 1497
JO - Biotechnology Letters
JF - Biotechnology Letters
IS - 10
ER -