Superresolution imaging and force characterization of optical tweezers using high-speed cameras

J. P. Staforelli, J. M. Brito, ESTEBAN MAURICIO VERA ROJAS, S. Torres, C. Saavedra

Resultado de la investigación: Capítulo del libro/informe/acta de congresoContribución a la conferenciarevisión exhaustiva

Resumen

The advantageous use of high speed cameras have been lately proposed for the direct characterization of optical tweezers in order to replace indirect measurements often performed by quadrant detectors, at the expense of a tradeoff between acquisition speed and resolution. In the proposed approach, subpixel motion data of the trapped particle is retrieved from a high speed low resolution video sequence. Due to the particular nature of the motion, we propose that the obtained motion information is not only useful for providing online characteristics of the optical trap force, but also for tackling the lack of resolution by means of applying superresolution algorithms on the low resolution image sequence. The results show that the proposed approach allows the characterization of the optical tweezers obtaining the real particle motion directly from the image domain, while still providing simultaneous high resolution imaging.

Idioma originalInglés
Título de la publicación alojadaFrontiers in Optics, FiO 2009
EditorialOptical Society of America (OSA)
ISBN (versión impresa)9781557528780
DOI
EstadoPublicada - 2009
Publicado de forma externa
EventoFrontiers in Optics, FiO 2009 - San Jose, CA, Estados Unidos
Duración: 11 oct 200915 oct 2009

Serie de la publicación

NombreOptics InfoBase Conference Papers
ISSN (versión digital)2162-2701

Conferencia

ConferenciaFrontiers in Optics, FiO 2009
País/TerritorioEstados Unidos
CiudadSan Jose, CA
Período11/10/0915/10/09

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