Two new species of Werneria (Compositae, Senecioneae) are described from the highlands of central Peru on the basis of morphological evidence, namely W. huascarana and W. rockhauseniana. In addition, the misinterpreted taxonomic entity W. weberbaueriana is properly circumscribed according to the protologue. A neotype is designated for the name W. weberbaueriana. A key to the Werneria species occurring in the Peruvian department of Ancash is also presented. When data are certain, conservation status is assessed. Citation: Calvo J., Trinidad H. & Beltrán H. 2020: Two new species of Werneria from Peru and re-circumscription of W. weberbaueriana (Compositae, Senecioneae).-Willdenowia 50: 5-12. doi: https://doi.org/10.3372/wi.50.50101 Version of record first published online on 21 February 2020 ahead of inclusion in April 2020 issue.